KMID : 0880220190570040238
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Journal of Microbiology 2019 Volume.57 No. 4 p.238 ~ p.242
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Mesorhizobium denitrificans sp. nov., a novel denitrifying bacterium isolated from sludge
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Siddiqi Muhammad Zubair
Thao Ngo Thi Phuong Choi Gyu-Min Kim Dae-Cheol Lee Young-Woo Kim Sang-Young Wee Ji-Hyang Im Wan-Taek
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Abstract
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A Gram-stain-negative, non-spore-forming, facultative, rod-shaped bacterium (designated LA-28T) was isolated from a sludge sample from a wastewater treatment plant in Hanam city, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain LA-28T clustered with species of the genus Mesorhizobium and appeared closely related to M. jarvisii LMG 28313T (96.8%), M. waimense ICMP 19557T (96.7%), and M. huakuii LMG 14107T (96.7%). Growth occurs at 18?40¡ÆC on R2A medium in the presence of 1?4% NaCl (w/v) and at pH 6?8. The DNA G+C content was 61.2 mol%, and the predominant quinone was ubiquinone-10 (Q-10). The major cellular fatty acids (> 5%) were C16:0, C19:0¥ø8c cyclo, C18:1¥ø7c 11-methyl, and C18:1¥ø7c and/or C18:1¥ø6c (summed feature 8). Major polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidyl-N-methylethanolamine (PME), and phosphatidylcholine (PC). Physiological and biochemical characteristics indicated that strain LA-28T represents a novel species of the genus Mesorhizobium, for which the name Mesorhizobium denitrificans sp. nov. is proposed. The type strain is LA-28T (= KACC 19675T = LMG 30806T).
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KEYWORD
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Mesorhizobium denitrificans, 16S rRNA gene sequence, polyphasic taxomony, sludge
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